Cannabis plant named ‘HAPPY PINEAPPLE’

ABSTRACT

The present invention provides new and distinct Cannabis cultivars designated as ‘HAPPY PINEAPPLE’. Disclosed herein are main terpenes of ‘HAPPY PINEAPPLE’, which are beta-ocimene, beta-caryophyllene, alpha-pinene, limonene, alpha-humulene, beta-pinene and myrcene. Also, the present invention provides the estimated concentration of the THCmax about 14.50-18.57% and CBDmax about 0.00%, respectively, at the time of assaying metabolites from flower samples of ‘HAPPY PINEAPPLE’.

Latin name of genus and species: Cannabis hybrid.

Variety denomination: ‘HAPPY PINEAPPLE’.

BACKGROUND OF THE INVENTION

The present invention relates to a new and distinct Cannabis cultivardesignated as ‘HAPPY PINEAPPLE’. This new cultivar is the result ofcontrolled-crosses between proprietary cultivars made by the inventors.The new cultivar of ‘HAPPY PINEAPPLE’ was asexually reproduced via astem ‘cutting’ and ‘cloning’ method by the inventors at Salinas, Calif.Asexual clones from the original source have been tested in greenhouses,nurseries, and/or fields. The properties of each cultivar were found tobe transmissible by such asexual reproduction. This cultivar is stableand reproduces true to type in successive generations of asexualreproduction.

TAXONOMY AND NOMENCLATURE

Cannabis, more commonly known as marijuana, is a genus of floweringplants that includes at least three species, Cannabis sativa, Cannabisindica and Cannabis ruderalis as determined by plant phenotypes andsecondary metabolite profiles. In practice however, Cannabisnomenclature is often used incorrectly or interchangeably. Cannabisliterature can be found referring to all Cannabis varieties as “sativas”or all cannabinoid producing plants as “indicas”. Indeed the promiscuouscrosses of indoor Cannabis breeding programs have made it difficult todistinguish varieties, with most Cannabis being sold in the UnitedStates having features of both sativa and indica species.

Human cultivation history of Cannabis dates back 8000 years (Schultes, RE., 1970, Random thoughts and queries on the botany of Cannabis. Pages11-38 in: CRB Joyce, and SH Curry eds., THE BOTANY AND CHEMISTRY OFCANNABIS. J. & A. Churchill. London, England). Hemp cloth recovered inEurope dates back 6000 years (Small, E, Beckstead, H D, and Chan, A,1975, The evolution of cannabinoid phenotypes in Cannabis, ECONOMICBOTANY 29(3):219-232). The written record of the pharmacologicproperties of Cannabis goes back more than 4000 years (Ti, H. 2737 BC.NEI JING SU WEN HUANG TI, Yellow Emperor's Classic on Internal Medicine;referred to without citation in Small et al. 1975 Supra).

The taxonomy and nomenclature of the highly variable genus Cannabis(Emboden, W A, 1974, ECONOMIC BOTANY 28(3):304-310; Small, E andCronquist, A, 1976, TAXON 25(4):405-435; Small E and Cronquist, A, 1977,TAXON 26(1): 110; Hillig, K W and Mahlberg, P G, 2004, American Journalof Botany 91(6):966-975), remains in question. This is in spite of thefact that its formal scientific name, Cannabis sativa L., assigned byCarolus Linneaus (Linnaeus, C, 1753, SPECIES PLANTARUM, 2:1027, Salvius,Stockholm, Facsimile edition, 1957-1959, Ray Society, London, U.K.), isone of the oldest established names in botanical history and is stillaccepted to this day. Another species in the genus, Cannabis indica Lam.was formally named somewhat later (de Lamarck, J B, 1785, ENCYCLOPEDIEMETHODIQUE DE BOTANIQUE, 1(2):694-695), but is still very old inbotanical history. In 1785, Jean-Baptiste Lamarck published adescription of a second species of Cannabis, which he named Cannabisindica. Lamarck based his description of the newly named species onplant specimens collected in India. C. indica was described asrelatively short, conical, and densely branched, whereas C. sativa wasdescribed as tall and laxly branched (Schultes R. E. et al, 1974,Harvard University Botanical Museum Leaflets, 23:337-367). C. indicaplants were also described as having short, broad leaflets whereas thoseof C. sativa were characterized as relatively long and narrow (AndersonL. C., 1980, Harvard University Botanical Museum Leaflets, 28:61-69). C.indica plants conforming to Schultes' and Anderson's descriptions mayhave originated from the Hindu Kush mountain range. Because of the oftenharsh and variable (extremely cold winters, and warm summers) climate ofthose parts, C. indica is well-suited for cultivation in temperateclimates.

Three other species names were proposed in the 1800s to distinguishplants with presumably different characteristics (C. macrosperma Stokes,C. chinensis Delile, C. gigantean Vilmorin), none of which are acceptedtoday, although the epithet “indica” lives on as a subspecies of C.sativa (C. sativa ssp. indica Lam., Small and Cronquist 1976 Supra).

In the 20th century, two new names were added to the liturgy of proposedCannabis species: C. ruderalis Janischevsky and a hybrid, x C. intersitaSojak. (Small, E, Jui, P Y, and Lefkovitch, L P, 1976, SYSTEMATIC BOTANY1(1): 67-84; Small and Cronquist 1976 Supra). Further, numerous nameshave been proposed for horticultural variants of Cannabis but as of1976, “very few of these have been validly published as formal taxaunder the International Code of Botanical Nomenclature” (Small andCronquist 1976 Supra). Moreover, other recent work continues to focus onhigher-order evolutionary relationships of the genus. Cannabis has beenvariously ascribed as belonging to mulberry family (Moraceae) (Engler, HG A, Ulmaceae, Moraceae and Urticaceae, pages 59-118 in: A. Engler andK. Prantl eds., 1889, DIE NATURLICHEN PFLANZENFAMILIEN 3(1). W.Engelmann, Leipzig, Germany; Judd, W S, Sanders, R W, and Donoghue, M J,1994, HARVARD PAPERS IN BOTANY 5:1-51; Humphries, C J and Blackmore, S,A review of the classification of the Moraceae, pages 267-277 In: Craneand Blackmore 1989 id.); nettle family (Urticaceae) (Berg, C C,Systematics and phylogeny of the Urticales, pages 193-220, in: P. R.Crane and S. Blackmore eds., 1989, EVOLUTION, SYSTEMATIC, AND FOSSILHISTORY OF THE HAMAMELIDAE, VOL. 2, HIGHER HAMAMELIDAE, Clarendon Press,Oxford, U.K.); and most recently in its own family with hops (Humulus),Cannabaceae, or hemp family (Sytsma, K J, et al, 2002, AMERICAN JOURNALOF BOTANY 89(9):1531-1546). While the work of Small and Cronquist 1976Supra, seemed to effectively confine the genus to a single species with2 subspecies (C. sativa ssp. sativa, C. sativa ssp. indica), each withtwo varieties (C. s. s. var. sativa, C. s. s. var. spontanea; C. s. i.var. indica, C. s. i. var. Kafiristanica) largely on the basis ofchemotaxonomy and interfertility of all forms, more recent work (Sytsmaet al. 2002 Supra), proposes a two-species concept, resurrecting thebinomial C. indica Lam. Since Sytsma et al. (2002) provides no key fordiscriminating between the species, the dichotomous key of Small andCronquist (1976), which accounts for all forms in nature, whether wildor domesticated, is preferred to classify the characteristics of theplants.

BRIEF SUMMARY OF THE INVENTION

This invention relates to a new and distinctive Cannabis cultivardesignated as ‘HAPPY PINEAPPLE’.

The objective of the breeding program which produced novel plantsdisclosed herein was primarily to develop a Cannabis cultivar with itsunique blend of various cannabinoids and/or terpenes for (a) medicinaleffects such as improving appetite and reducing nausea, vomiting and/orchronic pain, as well as neurological and cardiovascular effects, (b)psychoactive effects such as increased motivation and energetic behaviorrather than indifference, passiveness and lethargy, and (c) recreationaleffects with enhanced enjoyment such as food and aroma.

As used herein, the term “cultivar” is used interchangeably with“variety”, “strain”, and/or “clone”.

Cannabis plants produce a unique family of terpeno-phenolic compounds.Cannabinoids, terpenoids, and other compounds are secreted by glandulartrichomes that occur most abundantly on the floral calyxes and bracts offemale plants. As a drug it usually comes in the form of dried flowerbuds (marijuana), resin (hashish), or various extracts collectivelyknown as hashish oil. The Cannabis plant has at least 545 distinctcompounds that span 20 chemical classes including cannabinoids,terpenes, terpenoids, amino acids, nitrogenous compounds, simplealcohols, aldehydes, ketones, esters, lactones, acids, fatty acids,steroids, non-cannabinoid phenols, pigments, flavonoids, vitamins,proteins, enzymes, glycoproteins, and hydrocarbons. Terpenes and/orcannabinoids, in particular, have shown great potential in terms ofmedicinal value.

Terpenes and/or cannabinoids have been shown to be largely responsiblefor beneficial effects of a Cannabis plant. In fact, each Cannabis planthas the varying concentrations of medically viable compounds dependingon different strains (genotypes) and their resulting chemotypes. Even asmall variation in terpene and/or cannabinoid concentration can causenoticeable differences in the entourage and/or synergistic effects of aCannabis plant, which distinguishes one variety from another. Researchshows that it relies heavily on the physiological effects produced byterpenes and/or cannabinoids.

Over 100 different kinds of terpenes have been identified in Cannabisplants although not being as well-studied as cannabinoids, they areinstrumental in giving rise to the physiological and psychoactiveeffects in Cannabis.

Terpenes are a large and diverse class of organic compounds, produced bya variety of plants. They are often strong smelling and thus may havehad a protective function. Terpenes are an important component, not onlyinfluencing taste and smell of each Cannabis strain but also influencingits effects on the mind and body of a subject such as humans andanimals. Terpenes are a classification of organic molecules that arefound in a wide variety of plants and animals. These molecules are knownfor their characteristic scents and flavors. The varying terpeneconcentrations found in Cannabis plants directly influence the resultingtaste and smell, as well as the observed effects. Non-limiting examplesof terpenes include Hemiterpenes, Monoterpenes, Sesquiterpenes,Diterpenes, Sesterterpenes, Triterpenes, Sesquarterpenes, Tetraterpenes,Polyterpenes, and Norisoprenoids. The main terpenes found in Cannabisplants include, but are not limited to, myrcene, limonene,caryophyllene, pinene, terpinene, terpinolene, camphene, terpineol,phellandrene, carene, humulene, pulegone, sabinene, geraniol, linalool,fenchol, borneol, eucalyptol, and nerolidol.

Cannabinoids are the most studied group of the main physiologicallyactive secondary metabolites in Cannabis. The classical cannabinoids areconcentrated in a viscous resin produced in structures known asglandular trichomes. At least 113 different cannabinoids have beenisolated from Cannabis plants. The main classes of cannabinoids fromCannabis include tetrahydrocannabinol (THC), cannabidiol (CBD),cannabigerol (CBG), and cannabinol (CBN). Cannabinoid can be at leastone of a group comprising tetrahydrocannabinol (THC), cannabidiol (CBD),cannabigerol (CBG), cannabinol (CBN) cannabichromene (CBC),cannabinodiol (CBDL), cannabicyclol (CBL), cannabivarin (CBV),tetrahydrocannabivarin (THCV), cannabidivarin (CBDV), cannabigerovarin(CBGV), cannabichromevarin (CBCV), cannabigerol monomethyl ether (CBGM),cannabielsoin (CBE), cannabicitran (CBT), cannabinol propyl variant(CBNV), cannabitriol (CBO), tetrahydrocannabinolic acid (THCA),tetrahydrocannabivarinic acid (THCVA), cannabidiolic acid (CBDA),cannabigerolic acid (CBGA) and cannabinerolic acid.

Most cannabinoids exist in two forms, as acids and in neutral(decarboxylated) forms. The acidic form of cannabinoids is designated byan “A” at the end of its acronym (i.e. THCA). The cannabinoids in theiracidic forms (those ending in “-A”) can be converted to their non-acidicforms through a process called decarboxylation when the sample isheated. The phytocannabinoids are synthesized in the plant as acidicforms. While some decarboxylation does occur in the plant, it increasessignificantly post-harvest and the kinetics increase at hightemperatures (Flores-Sanchez and Verpoorte, 2008, Plant Cell Physiol.49(12): 1767-1782). The biologically active forms for human consumptionare the neutral forms. Decarboxylation is usually achieved by thoroughdrying of the plant material followed by heating it, often bycombustion, vaporization, heating, or baking in an oven. Unlessotherwise noted, references to cannabinoids in a plant include both theacidic and decarboxylated versions (e.g., CBD and CBDA).

The molecules lose mass through the process of decarboxylation. In orderto find the total theoretical active cannabinoids, the acid forms shouldbe multiplied by 87.7%. For example, THCA can be converted to active THCusing the formula: THCA×0.877=THC. The maximum THC for the sample is:THC_(max)=(THCA×0.877)+THC. This method has been validated according tothe principles of the International Conference on Harmonization.Similarly, CBDA can be converted to active CBD and the yield isdetermined using the yield formula: CBDA×0.877=CBD. Also the maximumamount of CBD yielded, i.e. max CBD for the sample is:CBD_(max)=(CBDA×0.877)+CBD. Additionally, CBGA can be converted toactive CBG by multiplying 87.8% to CBGA. Thus, the maximum amount of CBGis: CBG_(max)=(CBGA×0.878)+CBG.

The biologically active chemicals found in plants, phytochemicals, mayaffect the normal structure or function of the human body and in somecases treat disease. The mechanisms for the medicinal and psychoactiveproperties of a Cannabis plant, like any medicinal herb, produce thepharmacologic effects of its phytochemicals, and the key phytochemicalsfor a medical Cannabis plant are cannabinoids and terpenes.

Δ9-Tetrahydrocannabinol (THC) is a psychoactive cannabinoid responsiblefor many of the effects such as mild to moderate pain relief,relaxation, insomnia and appetite stimulation. THC has been demonstratedto have anti-depressant effects. The majority of strains range from12-21% THC with very potent and carefully prepared strains reaching evenhigher. While Δ9-Tetrahydrocannabinol (THC) is also implicated in thetreatment of disease, the psychotropic activity of THC makes itundesirable for some patients and/or indications.

Tetrahydrocannabinol, THC, is the primary psychoactive and medicinalcannabinoid and is the result of the decarboxylation oftetrahydrocannabinolic acid (THC-A), its acidic precursor. THC-A,(6ar,10ar)-1-hydroxy-6,6,9-trimethyl-3-pentyl-6a,7,8,10a-tetrahydro-6h-benzochromene-2-carboxylicacid, is found in the trichomes of the plant and converted into THC,which actually exists in only minute quantities in the living plant,after harvest and drying.

Cannabidiol (CBD) is one of the principal cannabinoids found in aCannabis plant and is largely considered to the most medicallysignificant. CBD occurs in many strains, at low levels, <1%. In somecases, CBD can be the dominant cannabinoid, as high as 15% by weight.CBD is non-psychoactive, meaning that unlike THC, CBD does not cause anoticeable “high”. CBD has shown potential for its medical properties inthe treatment of a wide variety of diseases and symptoms, includingcancer, nausea, chronic pain, spasms, seizures/epilepsy, anxiety,psoriasis, Crohn's disease, rheumatoid arthritis, diabetes,schizophrenia, post-traumatic stress disorder (PTSD), alcoholism,strokes, multiple sclerosis, and cardiovascular disease. CBD also hasbeen reported to act as a muscle relaxant, antibiotic,anti-inflammatory, and bone stimulant, as well as to improve bloodcirculation, cause drowsiness, and protect the nervous system. It canprovide relief for chronic pain due to muscle spasticity, convulsionsand inflammation, as well as effective relief from anxiety-relateddisorders. It can offer relief for patients with Multiple Sclerosis(MS), Fibromyalgia and Epilepsy. CBD has also been shown to inhibitcancer cell growth when injected into breast and brain tumors incombination with THC.

A Cannabis cultivar can be used to achieve the desire of patients to betreated with CBD without the adverse side-effects (e.g., psychoactivity)of THC.

Cannabichromene (CBC) is a rare, non-psychoactive cannabinoid, usuallyfound at low levels (<1%) when present. It has been shown to haveanti-depressant effects and to improve the pain-relieving effects ofTHC. Studies have demonstrated that CBC has sedative effects such aspromoting relaxation.

Cannabidiol (CBD) and cannabichromene (CBC) are both non-psychoactiveand end products of CBG metabolism, like THC, so that they can be usedmedically.

Cannabigerol (CBG) is a non-psychoactive cannabinoid. CBG-acid is theprecursor to both THC-acid and CBD-acid in the plant usually found atlow levels (<1%) when present. It has been demonstrated to have bothpain relieving and inflammation reducing effects. CBG reducesintraocular pressure, associated with glaucoma. CBG has been shown tohave antibiotic properties and to inhibit platelet aggregation, whichslows the rate of blood clotting. While Cannabigerol (CBG), is notconsidered psychoactive, it is known to block the psychoactive effectsof THC and is considered medically active in a variety of conditions.Its precursor, cannabigerolic acid, CBG-A,(E)-3-(3,7-Dimethyl-2,6-octadienyl)-2,4-dihydroxy-6-pentylbenzoic acid,is being studied medically.

Cannabinol (CBN) is an oxidative degradation product of THC. It mayresult from improper storage or curing and extensive processing, such aswhen making concentrates. It is usually formed when THC is exposed to UVlight and oxygen over time. CBN has some psychoactive properties, lessstrength than THC. CBN is thought to enhance the dizziness anddisorientation that users of Cannabis may experience. It may causefeelings of grogginess, and has been shown to reduce heart rate.

High potency Cannabis plants contain large quantities of specificterpenes as well as various assortments of other terpenes. For instance,a Cannabis plant may have a profile with either a high level of, amoderate amount of or a small amount of various terpenes depending onits cultivar and environmental conditions.

Various cultivars of ‘Cannabis’ species have been cultivated in aneffort to create a cultivar best suited to meet the interest ofinventors according to their own need. The particular plant disclosedherein was discovered in the area where the inventors were intentionallycross-pollinating and cultivating plants described below using standardMendelian breeding procedures well known to those of ordinary skill inthe art. This resulted in the progenies of the inventors' crosses.

The progenies resulting from any selection stage of either the crossing,selfing or backcrossing versions of the breeding regimes of the presentinvention were asexually reproduced to fix and maintain the desirableTHC content, CBs content, terpenes content, the aroma and flavor(s)typical of the desired class, and the other desirable phenotypic and/orgenotypic characteristics. The resultant selected Cannabis cultivar isdesignated as ‘HAPPY PINEAPPLE’ disclosed herein.

The inventors reproduced progenies asexually by stem cutting andcloning. This is the origin of this remarkable new cultivar. The planthas been and continues to be asexually reproduced by stem cutting andcloning at the inventors' greenhouses, nurseries and/or fields inSalinas, Calif., Oakland, Calif., and/or Washington, D.C.

The following are the most outstanding and distinguishing chemicalcharacteristics of this new cultivar when grown under normal conditionsin Salinas, Calif. Chemical analyses of the new Cannabis variety and thecheck variety (or the parental varieties) disclosed herein wereperformed using standard chemical separation techniques well known tothose skilled in the art. Samples for assaying were obtained from flowertissues of the Cannabis plant disclosed herein. Cannabinoid compositionof this cultivar can be determined by assaying the concentration of atleast one cannabinoid in a subset (e.g., sample) of the harvestedproduct.

Table 1 includes detailed information of the Cannabis plant named ‘HAPPYPINEAPPLE’ including the concentration ranges of terpenes andcannabinoids as tested on flowers at least four different times. TheCannabis plant has been tested in a laboratory setting and/or facilityto determine cannabinoids and terpenes concentrations in the Cannabisplant named ‘HAPPY PINEAPPLE’ according to the procedures provided inGiese et al. (Journal of AOAC International (2015) 98(6):1503-1522).

1) The main terpenes found in ‘HAPPY PINEAPPLE’ are beta-ocimene,beta-caryophyllene, alpha-pinene, limonene, alpha-humulene, beta-pineneand myrcene; and

2) The estimated concentration of the total THC_(max) and CBD_(max) isabout 14.50-18.57% and about 0.00%, respectively, at the time ofassaying metabolites from flower samples of ‘HAPPY PINEAPPLE’.

Terpene and cannabinoid profiles of ‘HAPPY PINEAPPLE’ demonstrate that‘HAPPY PINEAPPLE’ has a phenotypically unique profile, particularlyinsofar as to the level of terpenes and cannabinoids. This data ispresented in a tabular form in Table 1.

TABLE 1 Ranges of Active Cannabinoids and Terpenes Ranges of ActiveCannabinoids (% by weight) THC max 14.50-18.57% CBD max 0.00% Ranges ofTerpenes (% by weight) Terpinolene 0.00% Fenchol 0.02-0.06% Alphaphellandrene 0.00% Camphene 0.01-0.02% Beta ocimene 0.32-0.60% Alphaterpineol 0.03-0.05% Carene 0.00% Alpha humulene 0.08-0.18% Limonene0.14-0.32% Beta caryophyllene 0.20-0.48% Gamma terpinene 0.00% Linalool0.03-0.08% Alpha pinene 0.23-0.31% Caryophyllene oxide 0.01-0.02% Alphaterpinene 0.00% Myrcene 0.02-0.11% Beta pinene 0.08-0.11% Total Terpenes1.18-2.19%

The Cannabis plant named ‘HAPPY PINEAPPLE’ has a complement of terpenes,including but not limited to, relatively high levels of beta-ocimene,beta-caryophyllene, alpha-pinene, limonene, alpha-humulene, beta-pineneand myrcene compared to other terpene compounds. This unique combinationof differently concentrated terpenes further distinguishes ‘HAPPYPINEAPPLE’ from other varieties in its odor, its medical qualities, andits effects on mood and mentation.

Asexual Reproduction

Asexual reproduction, also known as “cloning”, is a process well knownto those of ordinary skill in the art of Cannabis production andbreeding and includes the following steps.

The Cannabis cultivar disclosed herein is asexually propagated viataking cuttings of shoots and putting them in rock wool cubes. Thesecubes are presoaked with pH-adjusted water and kept warm (˜80° F.). Fulltrays are covered, left under 18 hours of light and allowed to root(7-14 days). Upon root onset, the plantlets are transplanted into rigid1 gallon containers filled with a proprietary soil mix A and remain in18 hours of daylight for another 14-21 days. Once root-bound, plants aretransplanted into rigid 3 gallon containers filled with proprietary soilmix B. Immediately, the light cycle is altered to 12/12 and flowerinitiating begins. The plants remain in 12/12 lighting until harvesting.They undergo a propriety nutrient regimen and grow as undisturbed aspossible for 60-70 days depending on chemotype analysis.

All sun leaves are removed and the plant is dismantled to result inapproximately 12″ branches covered in inflorescences and trichomes. Thegoal in harvesting is to actually harvest trichome heads but not ‘buds’.Thus, great care is taken not to disturb the trichome heads and as muchof the plant remains intact as possible to promote even and slow drying.Slow drying is followed by a one to two months curing process.

Observation of the all female progenies of the original plant hasdemonstrated that this new and distinct cultivar has fulfilled theobjectives and that its distinctive characteristics are firmly fixed andhold true from generation to generation vegetatively propagated from theoriginal plant.

Under careful observation, the unique characteristics of the newcultivar have been uniform, stable and reproduced true to type insuccessive generations of asexual reproduction.

DESCRIPTION OF THE DRAWINGS

The accompanying color photographs depict characteristics of the newplants designated ‘HAPPY PINEAPPLE’ as nearly true as possible to makecolor reproductions. The overall appearance of the plants named ‘HAPPYPINEAPPLE’ in photographs is shown in colors that may differ slightlyfrom the color values described in the detailed botanical description.

FIG. 1A-C shows the ‘HAPPY PINEAPPLE’ plant at the mid to latevegetative growth stage; a close view of the middle part of plant fromthe side (FIG. 1A), a close view of the plant from the above (FIG. 1B),and another close view of the plant from the above (FIG. 1C).

FIG. 2 shows an overall view of the ‘HAPPY PINEAPPLE’ plant from theside. The large and tall plants in the back of FIG. 2 are the ‘HAPPYPINEAPPLE’ plants.

FIG. 3A shows a close view of a single leaf of the check variety BLK03plant.

FIG. 3B shows a close view of a single leaf of the new variety ‘HAPPYPINEAPPLE’ plant.

FIG. 4A shows top parts (including inflorescence) of the BLK03 plantfrom the side.

FIG. 4B shows top parts (including inflorescence) of the ‘HAPPYPINEAPPLE’ plant from the side.

FIG. 5 shows a close view of flowers of the ‘HAPPY PINEAPPLE’ plant atthe late flowering stage.

FIG. 6 shows a close view of flowers of the ‘HAPPY PINEAPPLE’ plant atthe late flowering stage.

FIG. 7 shows a close view of flowers of the ‘HAPPY PINEAPPLE’ plant atthe late flowering stage.

FIG. 8 shows a seed of the ‘HAPPY PINEAPPLE’ plant.

FIG. 9 shows a reproductive part of a sample Cannabis plant, indicatingposition/location of a flower, a bract and a stipule in the plant. Thesample Cannabis plant in FIG. 9 is not the claimed ‘HAPPY PINEAPPLE’plant.

DETAILED BOTANICAL DESCRIPTION

The ‘HAPPY PINEAPPLE’ plant has not been observed under all possibleenvironmental conditions, and the phenotype may vary significantly withvariations in environment. The following observations, measurements, andcomparisons describe this plant as grown at Salinas, Calif., when grownin the greenhouse, nursery or field, unless otherwise noted.

Plants for the botanical measurements in the present application areannual plants. In the following description, the color determination isin accordance with The Royal Horticultural Society Colour Chart, 2007,5^(th) Edition, except where general color terms of ordinary dictionarysignificance are used.

The Cannabis plant disclosed herein was derived from female and maleparents that are internally designated as below.

The internal GNBR code of the Cannabis cultivar named ‘HAPPY PINEAPPLE’is 39.R3.02. The internal GNBR Breeding Code of the Cannabis plant named‘HAPPY PINEAPPLE’ is (B03.S1.39)x(R08.S09.03).02. The additional number‘.02’ was only assigned to the 2^(nd) individual plant (i.e. ‘HAPPYPINEAPPLE’) selected for phenotypic and chemotypic traits from progeniesof the cross event between pollen acceptor (B03.S1.39) and pollen donor(R08.S09.03). ‘HAPPY PINEAPPLE’ is a fertile hybrid derived from acontrolled-cross between two proprietary cultivars: (i) B03.S1.39(pollen acceptor; female parent), also known as ‘B3.S1.39’ or ‘39’ and(ii) R08.S09.03 (pollen donor; male parent), also known as ‘R8.S9.03’ or‘R3’. The initial cross between two parental cultivars was made in Oct.28, 2016. The initial selection for the Cannabis cultivar named ‘HAPPYPINEAPPLE’ was made in Aug. 27, 2017. The primary phenotypic criteriaused to select the new and distinct Cannabis cultivar disclosed hereinis as follows: structure/phenolic(s) score, susceptibility/resistance topests, and susceptibility/resistance to diseases. Also, the chemotypiccharacteristics (including cannabinoids, terpenes, and other secondarymetabolites) described in Table 1 were used to select the new anddistinct Cannabis cultivar disclosed herein. The first asexualpropagation of ‘HAPPY PINEAPPLE’ occurred on Sep. 5, 2017 in Salinas,Calif.

The following traits in combination further distinguish the Cannabiscultivar ‘HAPPY PINEAPPLE’ from the check variety ‘BLK03’, which is setas a standard for phenotypic comparison. Tables 2 to 6 presentphenotypic traits and/or characteristics of ‘HAPPY PINEAPPLE’ comparedto the check variety ‘BLK03’ as follows. Unless otherwise indicated, allplants were raised together and evaluated at day 60 in flowering or atday 92 from when stem cuttings were placed in rooting media.

TABLE 2 General Characteristics Characteristics New Variety Plant lifeforms An herbaceous plant (herb) Plant growth An upright, tap-rootedannual habit plant Plant origin A controlled-cross between pollenacceptor (B03.S1.39) and (R08.S09.03) Plant Asexually propagated by stempropagation cuttings and cloning Propagation ease Easy Height 3.56-3.96m Width 2.13-2.24 m Plant vigor High Resistance to Pests that ‘HAPPYpests or diseases PINEAPPLE’ is resistant to: Two-spotted spider mite(Tetranychus urticae (Koch)); (2) Aphids species such as: CannabisAphids (Phorodon cannabis), Green Peach Aphid (Myzus persicae (Sulzer)),Foxglove Aphid (Aulacorthum solani), Peach Aphid (Macrosiphumeuphorbiae), Black Bean Aphid (Aphis fabae); (3) Whitefly (Trialeurodesvaporariorum; (4) Lepidoptera species such as: Armyworm (Spodopterafrupperda), Cabbage Whites (Pieris rapae), Painted Lady (Vanessacardui), Lepidoptera sp. Diseases that Happy Pineapple is resistant to:Powdery Mildew (Podosphaeraxanthii) Time to Harvest 8-10 weeks (Seed toHarvest) Genetically- NO modified organism Characteristics Check Variety(BLK03) Plant life forms An herbaceous plant (herb) Plant growth Anupright, tap-rooted annual habit plant Plant origin A controlled-crossbetween pollen acceptor (GLD13) and (BDIA) Plant Asexually propagated bystem propagation cuttings and cloning Propagation ease Moderate Height0.5-2.5 m Width 1.20 m Plant vigor Medium Resistance to Pests that BLK03is NOT pests or diseases resistant to: Two-spotted spider mite(Tetranychus urticae (Koch)); (2) Aphids species such as: CannabisAphids (Phorodon cannabis), Green Peach Aphid (Myzus persicae (Sulzer)),Foxglove Aphid (Aulacorthum solani), Peach Aphid (Macrosiphumeuphorbiae), Black Bean Aphid (Aphis fabae); (3) Whitefly (Trialeurodesvaporariorum; (4) Lepidoptera species such as: Armyworm (Spodopterafrupperda), Cabbage Whites (Pieris rapae), Painted Lady (Vanessacardui), Lepidoptera sp. Fungal diseases that BLK03 is NOT resistant to:Botrytis/Flower Rot (Botrytis cinerea) and Powdery Mildew (Podosphaeraxanthii) Time to Harvest 8 weeks (Seed to Harvest) Genetically- NOmodified organism

TABLE 3 Leaf/Foliage Characteristics New Variety Check Variety (BLK03)Leaf Opposite at seedling Opposite at seedling arrangement (immature)stage; (immature) stage; Alternate at flowering Alternate at flowering(mature) stage (mature) stage Leaf shape Palmately compound Palmatelycompound Leaf structure Linear-lanceolate Linear-lanceolate leafletblades leaflet blades with glandular hairs with glandular hairs Leafmargins Dentate, coarsely ser- Dentate, coarsely ser- rated, and theteeth point rated, and the teeth point away from the tip away from thetip Leaf hairs Present on both upper Present on both upper and lowersurfaces and lower surfaces Leaf length 33.5-39.5 cm 9.5-11.4 cm withpetiole at maturity Leaf width at 9.8-16.4 cm 9.6-12.4 cm maturity No.of leaflets 5-9 5-7 Middle largest 15.5-18.2 cm 1.8-4.2 cm (longest)leaflet length No. teeth of 35-38 21-28 middle leaflet Leaf (upper N186A(purple); 132A side) color 136A (green); Leaves (RHS No.) turn purplewhen temp- erature drops below 55 F. Leaf (lower 135D 134D side) color(RHS No.) Leaf glossiness Medium at the Strong at the upper leaf surfaceupper leaf surface Leaflet apex Lanceolate N/A shape Leaflet baseLanceolate N/A shape Vein/midrib Obliquely continuous Obliquelycontinuous shape throughout throughout leaflet leaflet Vein/midrib 139D144C color (RHS No.) Petiole length 13.4-18.3 cm 5.8-7.2 cm at maturityPetiole color 139D 150D (RHS No.) Intensity of Moderate-strongModerate-strong petiole anthocyanin Stipule shape Linear-lanceolateElliptical Stipule length 0.9-1.1 cm 0.4-0.8 cm at maturity Stipulecolor 139D 145C (RHS No.) Foliage Bitter, Acerbic, and N/A Fragranceearth-spice tones

TABLE 4 Stem Characteristics New Variety Check Variety (BLK03) Stemshape Hexagonal in shape, Hollow, ribbed, textured large, robust Stemdiameter 6.-8.1 cm 2.8-3.4 cm at base Stem color 149D 195C (RHS No.)Stem pith type Thick Absent Depth of main Medium Medium stemribs/grooves Internode length Base (7.0-11.5 cm); Base (4.5-6.1 cm);Canopy (4.0-11.0 cm) Canopy (3.0-6.9 cm)

TABLE 5 Inflorescence (Female/Pistillate Flowers) Characteristics NewVariety Flowering Cymes (compound in nature) (blooming) habitInflorescence Flowers develop above the apical position portions of mainand lateral axes Flower Cymose (Clusters of bracts are arrangementoverlapping within each cyme) Number of 200 flowers per cyme; 8-12flowers per panicles per plant plant Flower shape Urceolate (Eachindividual flower has a small green bract enclosing an ovary withtwo-long, slender stigmas projecting well above the bract) Flower1.04-1.21 cm (individual pistillate) length Flower 6.0-9.1 cm (compoundcyme) diameter Corolla shape No defined corolla Corolla Color N/A (RH SNo.) Bract shape Urceolate Bract size 3.1-6.0 mm Bract color N187A(purple); 134B (green) (RHS No.) Stigma shape Pointed, linearStigmalength 1.0-1.2 mm Stigma color 44A (RHS No.) Calyx shape Nodefined calyx Calyx color N/A (RHS No.) Trichome shape Capitate-stalkedglandular Trichome color 157A (early flowering stage); (RHS No.) N30B(late flowering stage) Other types of Capitate sessile trichomes aretrichomes present on the surfaces on leaves of plants, as well as beingnoticed in the flowers (color: 157A at day 40 in flowering). Duringlater flowering, i.e. day 55 to day 70 in flowering, the capitatestalked trichomes are present (color: N30B). Bulbous and non-glandulartrichomes are also present and most noticeable on the petioles, stems,and leaves (color: 157A). Terminal bud Oblong shape Terminal bud7.60-9.42 cm length Terminal bud 5.48-7.20 cm width Terminal budN187A(purple); 134B(green) color (RHS No.) Pedicel Present Pedicel color139D (RHS No.) Sepal Absent Sepal color N/A (RHS No.) Petal Absent(Apetulous) Petal color N/A (RH No.) Staminate shape No staminateflowers produced naturally; however, male flower (staminate) can beinduced with chemical compounds (such as silver nitrate and silverthiosulphate anionic complex). Pollen N/A Seed shape Globular andtextured Seed size 1.8-2.4 mm (Diameter) Seed color 199A (RHS No.)Marbling of Weak to absent seed Max THC About 14.50-18.57% content MaxCBD 0.00% content Characteristics Check Variety (BLK03) Flowering Cyme(blooming) habit Inflorescence Flowers develop above the apical positionportions of main and lateral axes Flower Cymose arrangement Number of50-70 flowers per cyme; flowers per 6-9 panicles per plant plant Flowershape Urceolate (Each individual flower has a small green bractenclosing an ovary with two-long, slender stigmas projecting well abovethe bract) Flower 0.85-1.09 cm (individual pistillate) length Flower4.7-8.1 cm (compound cyme) diameter Corolla shape No defined corollaCorolla Color N/A (RH S No.) Bract shape Urceolate Bract size 1.9-2.99mm Bract color 134A (RHS No.) Stigma shape Acute Stigmalength 1.3-1.6 mmStigma color 44A (RHS No.) Calyx shape No defined calyx Calyx color N/A(RHS No.) Trichome shape Capitate-stalked glandular Trichome color 157A(early flowering stage); (RHS No.) N30B (late flowering stage) Othertypes of Capitate sessile trichomes are trichomes present on thesurfaces on leaves of plants, as well as being noticed in the flowers(color: 157A at day 35 in flowering). During later flowering, i.e. day60 in flowering, capitate stalked trichomes are present (color: N30B).Bulbous and non-glandular trichomes are also present and most noticeableon the petioles, stems, and leaves (color: 157A). Terminal bud Oblongshape Terminal bud N/A length Terminal bud N/A width Terminal bud 134Acolor (RHS No.) Pedicel Present Pedicel color 150D (RHS No.) SepalAbsent Sepal color N/A (RHS No.) Petal Absent (Apetulous) Petal colorN/A (RH Staminate shape No staminate flowers produced naturally;however, male flower (staminate) can be induced with chemical compounds(such as silver nitrate and silver thiosulphate anionic complex). PollenN/A Seed shape Smooth and globular Seed size 1.6-1.9 mm (Diameter) Seedcolor 199D (RHS No.) Marbling of Weak to absent seed Max THC About18.88-19.37% content Max CBD 0.00% content N/A: Not available

TABLE 6 Other Characteristics Characteristics New Variety AromaCitrus-Pineapple Sweet Zest Proportion of N/A Hermaphrodite Time periodand 60-70 days condition of flowering/ blooming Plant Hardiness Hardy(120° F.-20° F.) Breaking action Strong, Flexible; resistant to breakageRooting rate High after cutting/cloning Types of Stem Cutting forCloning Shipping quality High, great Storage life Long (3-8 months withminor changes in physical appearance and/or smell/taste); Minor decreasein green coloration; Up to 8 months with minor change in metabolitesand/or appearance. Market use Medicinal Productivity of Weight per plantis approximately flower 0.68-1.59 kg (1.5-3.5 lbs) Characteristics CheckVariety (BLK03) Aroma Spice-Earth tones Proportion of N/A HermaphroditeTime period and 50-60 days condition of flowering/bloo ming PlantHardiness Hardy (120° F.-20° F.) Breaking action Stout, non-flexible;resistant to breakage Rooting rate Medium after cutting/cloning Types ofStem Cutting for Cloning Shipping quality Moderate Storage life Medium(2-6 months with minor changes in physical appearance and/ orsmell/taste) Market use N/A Productivity of Approximately 0.14-0.45 kgflower can be produced per plant; dependent on finished size; Growingconditions/environment will dictate final yield/output N/A: Notavailable

In general, ‘HAPPY PINEAPPLE’ is larger in width and height than bothparents, pollen acceptor (B03.S1.39) and pollen donor (R08.S09.03).‘HAPPY PINEAPPLE’ is more robust in terms of growing performance, timeto rooted clones, and time to flower maturity. As ‘HAPPY PINEAPPLE’ hasgreater resistance to pests and diseases as described in Table 2(specifically to powdery mildew and bud rot, i.e. Botrytis cinerea), ityields higher than both parents. Since ‘HAPPY PINEAPPLE’ has strongerbranches and thicker stems with greater flexibility than both parents,its main and lateral branches gives ‘HAPPY PINEAPPLE’ ability to producehigher yields under different growing conditions. The flowers of ‘HAPPYPINEAPPLE’ are larger in width and length than both parents. ‘HAPPYPINEAPPLE’ clearly demonstrates hybrid vigor, and outperforms bothparents overall. Chemically, ‘HAPPY PINEAPPLE’ has a higher cannabinoidcontent with a higher percentage of CBG-A content in addition to ahigher and unique/ terpene content and combination thereof than eitherparent.

When ‘HAPPY PINEAPPLE’ is compared to the check variety ‘BLK03’, ‘HAPPYPINEAPPLE’ is taller in plant height and wider in plant width than‘BLK03’. ‘HAPPY PINEAPPLE’ has higher plant vigor. ‘HAPPY PINEAPPLE’ haslonger leaves than ‘BLK03’ in terms of whole leaf length includingpetiole. Also, ‘HAPPY PINEAPPLE’ has longer leaflets than ‘BLK03’ whencomparing the length of middle largest leaflets. The petioles andstipules of ‘HAPPY PINEAPPLE’ are longer than those of ‘BLK03’ atmaturity. Regarding the stem diameter at base, ‘HAPPY PINEAPPLE’ is atleast twice longer than ‘BLK03’. Also, the internodes of ‘HAPPYPINEAPPLE’ are longer than that of ‘BLK03’ at both the base and thecanopy. When comparing the number of flowers per cyme, ‘HAPPY PINEAPPLE’have more flowers than ‘BLK03’. Regarding the compound cyme diameter,‘HAPPY PINEAPPLE’ is longer than ‘BLK03’, and also individual pistillateflowers of ‘HAPPY PINEAPPLE’ are longer than those of ‘BLK03’. ‘HAPPYPINEAPPLE’ has longer bracts than ‘BLK03’, while having a little shorterstigmas than ‘BLK03’. With respect to aroma, ‘HAPPY PINEAPPLE’ have asmell of citrus-pineapple with sweet zest, while ‘BLK03’ has a spicysmell with earth tones.

When ‘HAPPY PINEAPPLE’ is compared to the known Cannabis plant named‘ECUADORIAN SATIVA’ (U.S. Plant Pat. No. 27,475), there are severaldistinctive characteristics. For example, the overall form of ‘HAPPYPINEAPPLE’ plant is taller in height and wider across at the widestpoint than ‘ECUADORIAN SATIVA’ plant. ‘HAPPY PINEAPPLE’ plant has longerwhole leaves and middle leaflets at maturity than ‘ECUADORIAN SATIVA’plant. Also, ‘HAPPY PINEAPPLE’ plant has longer petioles than‘ECUADORIAN SATIVA’ plant. ‘HAPPY PINEAPPLE’ plant has longer stipulesthan the ‘ECUADORIAN SATIVA’ plant. Regarding stem diameter at base,‘HAPPY PINEAPPLE’ plant is at least twice longer than ‘ECUADORIANSATIVA’ plant. When comparing individual pistillate flower and cymelength, ‘HAPPY PINEAPPLE’ flowers are longer than ‘ECUADORIAN SATIVA’flowers. While the aroma of ‘ECUADORIAN SATIVA’ is strongly mephiticwith hints of limonene, ‘HAPPY PINEAPPLE’ has a smell ofcitrus-pineapple with sweet zest. When comparing total THC contentbetween ‘HAPPY PINEAPPLE’ and ‘ECUADORIAN SATIVA’, the total THC contentof ‘HAPPY PINEAPPLE’ is about 14.50-18.57%, while ‘ECUADORIAN SATIVA’accumulates 12.45% total THC.

The invention claimed is:
 1. A new and distinct cultivar of Cannabisplant named ‘HAPPY PINEAPPLE’ substantially as shown and describedherein.